Microbio- Growth requirements lab

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1. You set up an experiment where you grew E. coli at various temperatures. Explain how you will use turbidity to detect microbial growth?

2. Submit a graph that shows off the growth potential in different temperatures for your E. coli. Be sure to give the graph a title, and label the axis. In addition, answer the following questions about this experiment.
1) What is the optimal growth temperature for E. coli?
2)In accordance to the results you got, explain why or why not you believe that E. coli is a good human pathogen?

3. Submit a picture of E. coli in thioglycolate. In the space below indicate
1) what type of oxygen requirement category this bacteria falls into and
2) explain how you know.

4. Submit a picture of A. faecalis in thioglycolate. In the space below indicate
1) what type of oxygen requirement category this bacteria falls into and
2) explain how you know.

5. Submit a picture of C. sporegenes in thioglycolate. In the space below indicate
1) what type of oxygen requirement category this bacteria falls into and
2) explain how you know.

6. Briefly, state how phenol red and the Durham tube show metabolic abilities for the bacteria you used in the experiment. Be sure to state how the results correlate with this.

7. Line up your tubes of phenol red that you inoculated with bacteria (be sure that the Durham tube is visible) from left to right Glucose, Fructose, Sucrose. Take a picture of it and submit it to this question.Then answer the questions below:
1)What is the name of the pathogen.
2)Was this pathogen able to metabolize G, F, or S? Explain how you know (Don’t forget the Durham tube!)

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