Biochem and microbiology work

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First complete this biochem assignment Purpose:

• This assignment allows you to practice making up your own “test questions” as a study method for practicing your problem solving skills.
• This discussion format allows you and your peers to help check each other’s calculations, gives you additional problems for problems, and to get feedback on the clarity of your work and calculations (e.g., are your peers able to follow your logic and your calculations?)
• The ability to provide and respond to constructive feedback are skills that are critical for working with any team in laboratory, clinical, or administrative careers.

Task:

• On a piece of paper (or on your tablet), do the following:

1. 1. Choose ONE of the following amino acids (which have ionizable R-groups): Arg, Asp, Cys, Glu, His, Lys, Tyr
   2. Look up the structure of your AA and the three pKas (α-COOH, α-NH₃⁺, and the R-group) for your chosen amino acid.
   3. Write the equilibria equation for the dissociation of your amino acid. Draw out the structure of each ionization form (skeletal structures or Fisher projections are ok.)
   4. Label each ionization form with its net charge using the correct nomenclature (e.g., Gly⁺, Gly⁰, Gly^–)
   5. List/write down the pKas for the 3 ionizable groups (α-COOH, α-NH3+, and the R-group) for your chosen amino acid. Calculate the isoelectric point (pI).
   6. Choose a pH between 2-13 that is NOT equal to any of the pKas of your amino acid AND which we did not do in class nor on the study questions.
   7. Calculate the net charge on EACH ionizable group at this pH. Write out ALL your equations (e.g., Henderson-Hasselback) and calculations for the α-COOH, α-NH3+, and the R-group OR explain your justification briefly (e.g., when shortcut is used). Show all your work and/or calculations clearly in a step-wise manner so a reader can follow your logic and calculations.
   8. Calculate the TOTAL net charge for your amino acid at your chosen pH. Show your work and highlight or draw a box around your final answer.
   9. Calculate the ABSOLUTE net charge for your amino acid at your chosen pH. Show your work and highlight or draw a box around your final answer.

• See Table 4.1 in your textbook (also on p. 8 of your lecture notes) for the amino acid pKas. There are several questions in lecture notes and in the study questions/problems for this unit. Please also watch the Panopto recording of Calculating Net Charge for any AA and given pH.

THen please complete the attached documents (this exact documents don’t give me a new one) for week 6 please use this article https://pubmed.ncbi.nlm.nih.gov/35568033/